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CyFlow™ CD99 PE

CyFlow™ CD99 PE
Application: Flow cytometry
Laser: Blue, Green, Yellow
Alternative Name: E2, MIC2
Antigen: CD99
Isotype: IgG2a
Emission Maximum: 576 nm
Excitation Maximum: 496 nm, 565 nm
Clonality: monoclonal
Format/Fluorochrome: PE
Field of Interest: Immunophenotyping
Source Species: Mouse
Regulatory Status: RUO
Target Species: Human
Clone: 3B2/TA8
Order number: AT753952
HLDA Workshop HLDA VI—WS Code T 6T-097, BP 534 Quantity 100 tests Volume 1.0 mL... more
Product information "CyFlow™ CD99 PE"
HLDA WorkshopHLDA VI—WS Code T 6T-097, BP 534
Quantity100 tests
Volume1.0 mL
ImmunogenHuman thymocytes
Background InformationCD99 is a ubiquitous transmembrane type I sialoglycoprotein of a unique and poorly characterized protein family. CD99 is heavily O-glycosylated and was described as a T cell costimulator and strong activator of integrin-mediated actin cytoskeleton assembly, promoting cell adhesion and homotypic aggregation, immediate arrest on an inflamed vascular endothelium, and cell migration through it. Ligation of CD99 under some conditions can lead to apoptosis. Originally, CD99 was described as a human thymus leukemia antigen, an Ewing´s sarcoma-specific membrane marker, and an adhesion molecule involved in spontaneous rosette formation of T cells with erythrocytes.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
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