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CyFlow™ CD107a Alexa Fluor™ 488

CyFlow™ CD107a Alexa Fluor™ 488
Application: Flow cytometry
Clonality: monoclonal
Target Species: Human, Mouse, Non-Human Primates
Regulatory Status: RUO
Laser: Blue
Source Species: Mouse
Field of Interest: Immunophenotyping
Isotype: IgG1
Format/Fluorochrome: Alexa Fluor™ 488
Excitation Maximum: 495 nm
Emission Maximum: 519 nm
Antigen: CD107a
Alternative Name: LAMP-1
Clone: H4A3
Order number: CG942258
Quantity 100 tests Volume 0.4 mL Immunogen Human peripheral blood mononuclear cells... more
Product information "CyFlow™ CD107a Alexa Fluor™ 488"
Quantity100 tests
Volume0.4 mL
ImmunogenHuman peripheral blood mononuclear cells (PBMC)
Background InformationCD107a (LAMP-1; lysosome-associated membrane protein-1), together with CD107b (LAMP-2), is a major constituent of lysosomal membrane, 1-2 % of total CD107a is found also on the plasma membrane. The LAMP proteins are involved in lysosome biogenesis and are required for fusion of lysosomes with phagosomes. Increased CD107a immunoreactivity is observed in neurones, and in glial cells surrounding senile plaques in Alzheimers disease cases and is localized mainly in medullary epithelial cells, single macrophages and lymphocytes in acute thymic involution. CD107a is a good marker of mast cell activation.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.02% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
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