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CyFlow™ CD158d APC

CyFlow™ CD158d APC
Application: Flow cytometry
Isotype: IgG1
Alternative Name: 15.212, 103AS, KIR2DL4, KIR103, KIR103AS
Antigen: CD158d
Emission Maximum: 660 nm
Clonality: monoclonal
Excitation Maximum: 650 nm
Laser: Red
Format/Fluorochrome: APC
Field of Interest: Immunophenotyping
Source Species: Mouse
Regulatory Status: RUO
Target Species: Human
Clone: mAb#33
Order number: BN814356
Quantity 100 tests Volume 1.0 mL Immunogen NK3.3 cells and KIR2DL4-Ig fusion protein... more
Product information "CyFlow™ CD158d APC"
Quantity100 tests
Volume1.0 mL
ImmunogenNK3.3 cells and KIR2DL4-Ig fusion protein
Background InformationCD158d (KIR2DL4) is a KIR family member that shares structural features with both activating and inhibitory receptors and may mediate different functions under different circumstances. It contains cytoplasmic ITIM, suggesting inhibitory function, but also transmembrane domain similar to those of activating KIRs. It has been reported that CD158d serves as an inhibitory receptor for peripheral and uterine NK cells, but its ligation with soluble mAbs (unlike immobilized mAbs) results in activation of IFN-γ secretion. CD158d also binds both membrane form and soluble form of its ligand HLA-G.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
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