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CyFlow™ CD163 PerCP-Cy5.5

CyFlow™ CD163 PerCP-Cy5.5
Application: Flow cytometry
Excitation Maximum: 482 nm
Alternative Name: D11, GH1/61, M130, RM3/1
Antigen: CD163
Emission Maximum: 695 nm
Format/Fluorochrome: PerCP-Cy5.5
Clonality: monoclonal
Isotype: IgG1
Laser: Blue, Green
Field of Interest: Immunophenotyping
Source Species: Mouse
Regulatory Status: RUO
Target Species: Human
Clone: GHI/61
Order number: BR471109
HLDA Workshop HLDA VI—WS Code M38 Quantity 100 tests Volume 0.4 mL Immunogen... more
Product information "CyFlow™ CD163 PerCP-Cy5.5"
HLDA WorkshopHLDA VI—WS Code M38
Quantity100 tests
Volume0.4 mL
ImmunogenHairy cell leukemia cells
Background InformationCD163 (M130) is a member of the scavenger receptor family, accounting for the clearance of hemoglobin-haptoglobin complexes during limited hemolysis, which protects the body, in particular the kidneys, against heme-mediated oxidative damages. It does not have measurable affinity for noncomplexed hemoglobin or haptoglobin. Immunomodulatory role of CD163 has been postulated. CD163 is expressed by cells of the monocyte-macrophage lineage and its extracellular part also circulates in plasma as a soluble protein, especially during sepsis and other conditions affecting macrophage activity, when its level may raise manyfold.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
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