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CyFlow™ CD177 Pacific Blue™

Application:	Flow cytometry
Emission Maximum:	455 nm
Alternative Name:	HNA-2a, HNA2A, NB-1, NB1, PRV1
Antigen:	CD177
Target Species:	Human, Non-Human Primates
Excitation Maximum:	400 to 410 nm
Clonality:	monoclonal
Laser:	Violet
Format/Fluorochrome:	Pacific Blue™
Isotype:	IgG1
Field of Interest:	Immunophenotyping
Source Species:	Mouse
Regulatory Status:	RUO
Clone:	MEM-166
Order number:	BR642789

Quantity 100 tests Volume 0.4 mL Immunogen Human granulocytes Background... more

Product information "CyFlow™ CD177 Pacific Blue™"

Quantity	100 tests
Volume	0.4 mL
Immunogen	Human granulocytes
Background Information	CD177 (NB1, HNA-2a and PRV-1 form) is a GPI-anchored glycoprotein present mainly on neutrophils. Its plasma membrane expression is increased during pregnancy and and inflammation or after G-CSF application. Ligand of CD177 has been identified as CD31 (PECAM-1). CD177 participates in neutrophil transmigration and seems to be also a pro-proliferative molecule. The antibodies against CD177 can be involved in neonatal alloimmune neutropenia (NAN).
Usage	The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage Buffer	The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA.
Storage	Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
Stability	Do not use after expiration date stamped on vial label.

Safety Data Sheet

Technical Data Sheet

CyFlow CD177 Pacific Blue (MEM-166) BR642789 TDS RUO.pdf

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