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CyFlow™ CD180 PE
| Application: | Flow cytometry |
| Excitation Maximum: | 496 nm, 565 nm |
| Alternative Name: | Bgp95, Ly64, RP105 |
| Antigen: | CD180 |
| Isotype: | IgG1 |
| Emission Maximum: | 576 nm |
| Laser: | Blue, Green, Yellow |
| Clonality: | monoclonal |
| Format/Fluorochrome: | PE |
| Field of Interest: | Immunophenotyping |
| Source Species: | Mouse |
| Regulatory Status: | RUO |
| Target Species: | Human |
| Clone: | G28-8 |
| Order number: | BJ916358 |
For Research Use Only
Not for use in diagnostic or therapeutic procedures.
Quantity 100 tests Volume 2.0 mL Immunogen Human tonsillar B cells Background... more
Product information "CyFlow™ CD180 PE"
| Quantity | 100 tests |
| Volume | 2.0 mL |
| Immunogen | Human tonsillar B cells |
| Background Information | CD180 (RP105, Bgp95, LY64) is a type I membrane glycoprotein of Toll-like receptor (TLR) family. Its cytoplasmic tail is short and unlike the TLRs, it lacks the TIR domain. CD180 expression is dependent on the coexpression of its helper molecule, MD-1, and mirrors that of TLR4 on antigen-presenting cells. CD180 regulates recognition of LPS and signaling in B cells, via interacting directly with the TLR4 signaling complex, inhibiting its ability to bind microbial ligands. Ligation of CD180 by monoclonal antibodies leads to B cell activation, upregulation of CD80/CD86, and increase in cell size. |
| Usage | The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 20·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests. |
| Storage Buffer | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
Safety Data Sheet
Technical Data Sheet
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