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CyFlow™ CD180 Low Endotoxin

CyFlow™ CD180 Low Endotoxin
Application: Flow cytometry, Functional assays, Immunoprecipitation
Clonality: monoclonal
Target Species: Human
Regulatory Status: RUO
Source Species: Mouse
Field of Interest: Immunophenotyping
Isotype: IgG1
Format/Fluorochrome: Low Endotoxin
Antigen: CD180
Alternative Name: Bgp95, Ly64, RP105
Clone: G28-8
Order number: CP389435
Concentration Unit mg/mL Concentration 1 Quantity 0.1 mg Volume 0.1 mL... more
Product information "CyFlow™ CD180 Low Endotoxin"
Concentration Unitmg/mL
Concentration1
Quantity0.1 mg
Volume0.1 mL
ImmunogenHuman tonsillar B cells
Background InformationCD180 (RP105, Bgp95, LY64) is a type I membrane glycoprotein of Toll-like receptor (TLR) family. Its cytoplasmic tail is short and unlike the TLRs, it lacks the TIR domain. CD180 expression is dependent on the coexpression of its helper molecule, MD-1, and mirrors that of TLR4 on antigen-presenting cells. CD180 regulates recognition of LPS and signaling in B cells, via interacting directly with the TLR4 signaling complex, inhibiting its ability to bind microbial ligands. Ligation of CD180 by monoclonal antibodies leads to B cell activation, upregulation of CD80/CD86, and increase in cell size.
Storage BufferThe reagent is provided in azide-free phosphate buffered saline (PBS) solution, pH ≈7.4; 0.2 µm filter sterilized. Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
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