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CyFlow™ CD229 APC

CyFlow™ CD229 APC
Application: Flow cytometry
Emission Maximum: 660 nm
Alternative Name: hly9, Ly-9, mLY9, SLAMF3
Antigen: CD229
Isotype: IgG1
Excitation Maximum: 650 nm
Clonality: monoclonal
Laser: Red
Format/Fluorochrome: APC
Field of Interest: Immunophenotyping
Source Species: Mouse
Regulatory Status: RUO
Target Species: Human
Clone: HLy9.25
Order number: CH984515
Quantity 100 tests Volume 1.0 mL Immunogen CD299-transfected 300.19 pre-B cell line... more
Product information "CyFlow™ CD229 APC"
Quantity100 tests
Volume1.0 mL
ImmunogenCD299-transfected 300.19 pre-B cell line
Background InformationCD229 (Ly9) is a cell surface receptor of the CD150 family, which includes also e.g. CD48 and CD224. Receptors of this family regulate cytokine production and cytotoxicity of lymphocytes and NK cells. High levels of CD229 are found on T and B cells, where its expression increases during their maturation. It is absent on granulocytes, bone marrow-derived dendritic cells, platelets and erythrocytes. CD229 has been also reported on mouse monocytes and NK cells. CD229 interacts homophilically through its N-terminal domain and localizes to the contact site between T cells and antigen presenting B cells during antigen-dependent immune synapse formation.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
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