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CyFlow™ CD300a PE

CyFlow™ CD300a PE
Application: Flow cytometry
Excitation Maximum: 496 nm, 565 nm
Alternative Name: CMRF35H, IGSF12, IRC1, IRp60
Antigen: CD300a
Isotype: IgG1
Emission Maximum: 576 nm
Laser: Blue, Green, Yellow
Clonality: monoclonal
Format/Fluorochrome: PE
Field of Interest: Immunophenotyping
Source Species: Mouse
Regulatory Status: RUO
Target Species: Human
Clone: MEM-260
Order number: CK802473
Quantity 100 tests Volume 2.0 mL Immunogen Human Acute Lymphocytic Leukemia (ALL) T... more
Product information "CyFlow™ CD300a PE"
Quantity100 tests
Volume2.0 mL
ImmunogenHuman Acute Lymphocytic Leukemia (ALL) T cells
Background InformationCD300a (CMRF-35H, IRp60) is a non-MHC-specific inhibitory receptor of immunoglobulin superfamily, which contains three immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that associate with SH2-containing phosphatases SHP-1 and SHP-2. CD300a is expressed on many cell types including T cells, NK cells, neutrophils, eosinophils or mast cells. Its triggering inhibits activating signals such as those of IL5, GM-CSF or eotaxin, as well as supresses mast cell degranulation or NK cell cytotoxic activity.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 20·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
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