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CyFlow™ CD314 FITC

CyFlow™ CD314 FITC
Application: Flow cytometry
Isotype: IgG1
Alternative Name: KLAR, KLR, KLRK1, NKG2-D, NKG2D
Antigen: CD314
Field of Interest: Immunophenotyping
Clonality: monoclonal
Source Species: Mouse
Emission Maximum: 518 nm
Excitation Maximum: 490 to 495 nm
Laser: Blue
Regulatory Status: RUO
Target Species: Human
Format/Fluorochrome: FITC
Clone: 1D11
Order number: BJ615099
Quantity 100 tests Volume 0.4 mL Immunogen NKL cell line Background Information... more
Product information "CyFlow™ CD314 FITC"
Quantity100 tests
Volume0.4 mL
ImmunogenNKL cell line
Background InformationCD314 (NKG2D; natural killer receptor G2D or KLRK1; killer cell lectin-like receptor subfamily K, member 1), is a homodimeric C-type lectin-like activating receptor and costimulator with type II membrane orientation (C teminus extracellular). CD314 homodimers are associated with DAP10, a membrane adaptor protein that signals similar to CD28 by recruitment of phosphatidylinositol 3-kinase. Engagement of CD314 amplifies antigen-specific T cell responses in CD314-positive T cell populations. In NK cells, CD314 is a primary activating receptor. As CD314 ligands the MHC class-I chain-related proteins A and B (MICA, MICB) and UL16-binding proteins (ULBPs) have been identified.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
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