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CyFlow™ IgE FITC
Application: | Flow cytometry |
Clonality: | monoclonal |
Format/Fluorochrome: | FITC |
Target Species: | Human |
Regulatory Status: | RUO |
Laser: | Blue |
Excitation Maximum: | 490 to 495 nm |
Emission Maximum: | 518 nm |
Source Species: | Mouse |
Isotype: | IgG1 |
Field of Interest: | Immunoglobulins |
Antigen: | IgE |
Clone: | BE5 |
Order number: | CM987907 |
For Research Use Only
Not for use in diagnostic or therapeutic procedures.
Concentration Unit mg/mL Concentration 0,1 Quantity 0.1 mg Volume 1.0 mL... more
Product information "CyFlow™ IgE FITC"
Concentration Unit | mg/mL |
Concentration | 0,1 |
Quantity | 0.1 mg |
Volume | 1.0 mL |
Immunogen | Purified human IgE |
Background Information | Immunoglobulin E (IgE) is a 180 kDa soluble protein serving as an antigen-specific unit of mast cell effector mechanisms. IgE has the lowest serum concentration of all immunoglobulins (approximately 0.5 mg/l) in healthy individuals, but upon allergen challenge its concentration in blood increases dramatically. Although biological survival of free IgE is very short (T1/2 = 2 days), it is stabilized after binding to its high affinity receptor. Unlike IgM- IgG- and IgA-committed B cells, IgE-switched B cells do not undergo clonal expansion. |
Usage | The reagent is designed for Flow Cytometry analysis. Suggested working usage is 5·µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator. |
Storage Buffer | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide. |
Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
Stability | Do not use after expiration date stamped on vial label. |
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