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CyFlow™ Ki-67 Alexa Fluor™ 488
Application: | Flow cytometry |
Clonality: | monoclonal |
Target Species: | Cow, Human |
Regulatory Status: | RUO |
Laser: | Blue |
Source Species: | Mouse |
Isotype: | IgG1 |
Format/Fluorochrome: | Alexa Fluor™ 488 |
Excitation Maximum: | 495 nm |
Emission Maximum: | 519 nm |
Field of Interest: | Transcription |
Antigen: | Ki-67 |
Clone: | Ki-67 |
Order number: | BN097991 |
For Research Use Only
Not for use in diagnostic or therapeutic procedures.
Quantity 100 tests Volume 0.4 mL Immunogen Nuclei of the Hodgkin lymphoma cell line... more
Product information "CyFlow™ Ki-67 Alexa Fluor™ 488"
Quantity | 100 tests |
Volume | 0.4 mL |
Immunogen | Nuclei of the Hodgkin lymphoma cell line L428 |
Background Information | Ki-67 is a highly protease-sensitive nuclear protein expressed in two isoforms (345 kDa and 395 kDa), both of which are identified by the antibody clone Ki-67. The Ki-67 antigen is essential for cell proliferation and its expression is restricted to the cycling cells. It is detected in G1, S, G2 and M phase, whereas it is absent in cells which are in G0 phase and it is not associated with DNA repair processes. Ki-67 thus represents an important tool for detection of proliferating cells, which is of great importance in tumor diagnostics and is commonly used as a prognostic factor in cancer studies. |
Usage | The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. |
Storage Buffer | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA. |
Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
Stability | Do not use after expiration date stamped on vial label. |
Safety Data Sheet
Technical Data Sheet
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