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CyFlow™ STRO1 PE

CyFlow™ STRO1 PE
Application: Flow cytometry
Clonality: monoclonal
Target Species: Human
Regulatory Status: RUO
Laser: Blue, Green, Yellow
Source Species: Mouse
Field of Interest: Immunophenotyping
Format/Fluorochrome: PE
Excitation Maximum: 496 nm, 565 nm
Emission Maximum: 576 nm
Isotype: IgM
Antigen: STRO1
Clone: STRO-1
Order number: BA518386
Concentration Unit mg/mL Concentration 0,1 Quantity 0.1 mg Volume 1.0 mL... more
Product information "CyFlow™ STRO1 PE"
Concentration Unitmg/mL
Concentration0,1
Quantity0.1 mg
Volume1.0 mL
ImmunogenHuman CD34 positive bone marrow cells
Background InformationSTRO-1 is a cell surface antigen expressed by stromal elements in human bone marrow, identified by monoclonal antibody STRO1. Approximately 10% of mononuclear cells, greater than 95% of which are nucleated erythroid precursors, are STRO-1 positive, whereas the CFU-GM (colony-forming unit granulocyte-macrophage), BFU-E (erythroid burst) and CFU-Mix (mixed colonies) committed progenitor cells are negative. CFU-F (fibroblast colony-forming cells) are present exclusively in the STRO-1 positive population. When plated under long-term bone marrow culture conditions, STRO-1 positive cells generate adherent cell layers containing multiple stromal cell types, including adipocytes, smooth muscle cells, osteoblasts, chondrocytes, and fibroblastic elements. In combination with glycophorin A, STRO-1 is a useful marker for identification of mesenchymal stem cells. STRO-1 and CD117 are markers for osteosarcoma cells.
UsageThe reagent is designed for Flow Cytometry analysis. Working concentrations should be determined by the investigator.
Storage BufferThe reagent is provided in stabilizing Tris buffered saline (TBS) solution, pH ≈8.0, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
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