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CyFlow™ CD2 Pacific Blue™

CyFlow™ CD2 Pacific Blue™
Application: Flow cytometry
Clonality: monoclonal
Target Species: Human
Regulatory Status: RUO
Isotype: IgG2b
Source Species: Mouse
Field of Interest: Immunophenotyping
Antigen: CD2
Alternative Name: LFA-2
Clone: LT2
Format/Fluorochrome: Pacific Blue™
Laser: Violet
Excitation Maximum: 400 to 410 nm
Emission Maximum: 455 nm
Order number: CX149909

For Research Use Only
Not for use in diagnostic or therapeutic procedures.

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Description
HLDA Workshop HLDA VI—WS Code T 6T-008 Quantity 100 tests Volume 0.4 mL Immunogen... more
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Product information "CyFlow™ CD2 Pacific Blue™"
HLDA WorkshopHLDA VI—WS Code T 6T-008
Quantity100 tests
Volume0.4 mL
ImmunogenNormal human blood lymphocytes
Background InformationCD2 (LFA-2) belongs to T lymphocyte glycoproteins of immunoglobulin superfamily. Its interaction with CD58 stabilizes adhesion between T cells and antigen presenting or target cells. Relatively low affinity of CD2 to CD58 (as measured in solution) is compensated within the two-dimensional cell-cell interface to provide tight adhesion. Moreover, T cell activation induces increased CD2 expression and its lateral mobility, making easier contact between CD2 and CD58. Subsequently, T cell activation causes fixation of CD58-CD2 at sites of cell-cell contact, thereby strengthening intercellular adhesion. CD2 deficiency reduces intestinal inflammation and helps to control infection.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
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