CyFlow™ CD3 Low Endotoxin

Antigen:	CD3
Alternative Name:	Leu4, T3
Clone:	MEM-92
Application:	Flow cytometry, Functional assays, Immunoprecipitation
Format/Fluorochrome:	Low Endotoxin
Target Species:	Human
Field of Interest:	Immunophenotyping
Species of Origin:	Mouse
Regulatory Status:	RUO
Clonality:	monoclonal
Isotype:	IgM
Order number:	BB372557

For Research Use Only
Not for use in diagnostic or therapeutic procedures.

HLDA Workshop HLDA IV—WS Code T 97 Concentration Unit mg/mL Concentration 1... more

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CyFlow™ CD3 Low Endotoxin

HLDA Workshop	HLDA IV—WS Code T 97
Concentration Unit	mg/mL
Concentration	1
Quantity	0.1 mg
Volume	0.1
Immunogen	Human thymocytes and T lymphocytes
Background Information	CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3γ, CD3 δ, CD3ε and CD3ζ. These CD3 subunits are structurally related members of the immunoglobulins superfamily encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
Storage Buffer	The reagent is provided in azide-free phosphate buffered saline (PBS) solution, pH ≈7.4; 0.2 µm filter sterilized. Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.
Storage	Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
Stability	Do not use after expiration date stamped on vial label.

Specific References

| Knapp W, Dorken B, Gilks W, Rieber EP, Schmidt RE, Stein H, von dem Borne AEGK (Eds): Leucocyte Typing IV. Oxford·University·Press,·Oxford. 1989; 1‑1820. <·NLM·ID:·8914679·> | Soucek J, Hilgert I, Budová I, Lindnerová G: Augmentation of NK cell activity and proliferation in cultured lymphocytes of leukemic patients by monoclonal antibodies CD3 and interleukin‑2. Neoplasma. 1994; 41(2):75‑81. <·PMID:·8208318·> | Stefanová I, Saville MW, Peters C, Cleghorn FR, Schwartz D, Venzon DJ, Weinhold KJ, Jack N, Bartholomew C, Blattner WA, Yarchoan R, Bolen JB, Horak ID: HIV infection‑‑induced posttranslational modification of T cell signaling molecules associated with disease progression. J·Clin·Invest. 1996·Sep·15; 98(6):1290‑7. <·PMID:·8823293·> | Brdicka T, Imrich M, Angelisova P, Brdickova N, Horvath O, Spicka J, Hilgert I, Luskova P, Draber P, Novak P, Engels N, Wienands J, Simeoni L, Osterreicher J, Aguado E, Malissen M, Schraven B, Horejsi V: Non‑T cell activation linker (NTAL): a transmembrane adaptor protein involved in immunoreceptor signaling. J·Exp·Med. 2002·Dec·16; 196(12):1617‑26. <·PMID:·12486104·> | Brdicková N, Brdicka T, Angelisová P, Horváth O, Spicka J, Hilgert I, Paces J, Simeoni L, Kliche S, Merten C, Schraven B, Horejsí V: LIME: a new membrane Raft‑associated adaptor protein involved in CD4 and CD8 coreceptor signaling. J·Exp·Med. 2003·Nov·17; 198(10):1453‑62. <·PMID:·14610046·> | Stroncek DF, Caruccio L, Bettinotti M: CD177: A member of the Ly‑6 gene superfamily involved with neutrophil proliferation and polycythemia vera. J·Transl·Med. 2004·Mar·29; 2(1):8. <·PMID:·15050027·> | Batista A, Millán J, Mittelbrunn M, Sánchez-Madrid F, Alonso MA: Recruitment of transferrin receptor to immunological synapse in response to TCR engagement. J·Immunol. 2004·Jun·1; 172(11):6709‑14. <·PMID:·15153487·> | Huang Y, Wange RL: T cell receptor signaling: beyond complex complexes. J·Biol·Chem. 2004·Jul·9; 279(28):28827‑3. <·PMID:·15084594·> | Kuhns MS, Davis MM, Garcia KC: Deconstructing the form and function of the TCR/CD3 complex. Immunity. 2006·Feb; 24(2):133‑9. <·PMID:·16473826·> | Alarcón B, Swamy M, van Santen HM, Schamel WW: T‑cell antigen‑receptor stoichiometry: pre‑clustering for sensitivity. EMBO·Rep. 2006·May; 7(5):490‑5. <·PMID:·16670682·> | Meraner P, Horejsí V, Wolpl A, Fischer GF, Stingl G, Maurer D: Dendritic cells sensitize TCRs through self‑MHC‑mediated Src family kinase activation. J·Immunol. 2007·Feb·15; 178(4):2262‑71. <·PMID:·17277131·>

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