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CyFlow™ CD3 act. PE
Application: | Flow cytometry |
Clonality: | monoclonal |
Target Species: | Human, Mouse |
Regulatory Status: | RUO |
Laser: | Blue, Green, Yellow |
Source Species: | Mouse |
Field of Interest: | Immunophenotyping |
Format/Fluorochrome: | PE |
Excitation Maximum: | 496 nm, 565 nm |
Emission Maximum: | 576 nm |
Isotype: | IgG1 |
Antigen: | CD3 (activation epitope) |
Clone: | APA1/1 |
Order number: | CM152332 |
For Research Use Only
Not for use in diagnostic or therapeutic procedures.
HLDA Workshop HLDA III—WS Code T 126 Concentration Unit mg/mL Concentration 0,1... more
Product information "CyFlow™ CD3 act. PE"
HLDA Workshop | HLDA III—WS Code T 126 |
Concentration Unit | mg/mL |
Concentration | 0,1 |
Quantity | 0.1 mg |
Volume | 1.0 mL |
Immunogen | Purified human CD3 proteins isolated from thymus |
Background Information | CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3γ, CD3 δ, CD3ε and CD3ζ. These CD3 subunits are structurally related members of the immunoglobulins superfamily encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. |
Usage | The reagent is designed for Flow Cytometry analysis. Suggested working usage is 5·µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator. |
Storage Buffer | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide. |
Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
Stability | Do not use after expiration date stamped on vial label. |
Safety Data Sheet
Technical Data Sheet
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