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CyFlow™ CD8 Pacific Blue™

CyFlow™ CD8 Pacific Blue™
Application: Flow cytometry
Clonality: monoclonal
Target Species: Human
Regulatory Status: RUO
Source Species: Mouse
Field of Interest: Immunophenotyping
Format/Fluorochrome: Pacific Blue™
Laser: Violet
Excitation Maximum: 400 to 410 nm
Emission Maximum: 455 nm
Isotype: IgG2a
Antigen: CD8
Clone: MEM-31
Order number: AY490430
HLDA Workshop HLDA III—WS Code T 575 Quantity 100 tests Volume 0.4 mL Immunogen... more
Product information "CyFlow™ CD8 Pacific Blue™"
HLDA WorkshopHLDA III—WS Code T 575
Quantity100 tests
Volume0.4 mL
ImmunogenCrude thymus membrane fraction
Background InformationThe CD8 T cell coreceptor (monomer approx. 32-34 kDa) is expressed as α/β heterodimer on majority of MHC I-restricted conventional T cells and thymocytes and as α/α homodimer on subsets of memory T cells, intraepithelial lymphocytes, NK cells and dendritic cells. Regulation of CD8 β level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 α-β but not α-α dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Safety Data Sheet

Technical Data Sheet

Instruction for Use

ECDoC

Certificate Of Analysis

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