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CyFlow™ CD14 Pacific Orange™
| Application: | Flow cytometry |
| Clonality: | monoclonal |
| Target Species: | Human, Non-Human Primates |
| Regulatory Status: | RUO |
| Source Species: | Mouse |
| Field of Interest: | Immunophenotyping |
| Isotype: | IgG1 |
| Laser: | Violet |
| Format/Fluorochrome: | Pacific Orange™ |
| Excitation Maximum: | 400 nm |
| Emission Maximum: | 551 nm |
| Antigen: | CD14 |
| Alternative Name: | LPS-R |
| Clone: | MEM-15 |
| Order number: | AJ589037 |
For Research Use Only
Not for use in diagnostic or therapeutic procedures.
HLDA Workshop HLDA III—WS Code M 252 HLDA IV—WS Code N 90 HLDA IV—WS Code T 53 HLDA V—WS Code... more
Product information "CyFlow™ CD14 Pacific Orange™"
| HLDA Workshop | HLDA III—WS Code M 252 HLDA IV—WS Code N 90 HLDA IV—WS Code T 53 HLDA V—WS Code M MA086 HLDA VI—WS Code M MA94 |
| Quantity | 100 tests |
| Volume | 0.4 mL |
| Immunogen | A crude mixture of human urinary proteins precipitated by ammonium sulphate from the urine of a patient suffering from proteinuria |
| Background Information | CD14 (LPS-R) is a 55 kDa GPI-anchored glycoprotein, constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils, where serves as a multifunctional lipopolysaccharide receptor; it is also released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 is able to discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. |
| Usage | The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. |
| Storage Buffer | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
Safety Data Sheet
Technical Data Sheet
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