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CyFlow™ CD14 PE-DyLight™ 594

CyFlow™ CD14 PE-DyLight™ 594
Application: Flow cytometry
Isotype: IgG1
Alternative Name: LPS-R
Antigen: CD14
Emission Maximum: 618 nm
Excitation Maximum: 593 nm
Format/Fluorochrome: PE-DyLight™ 594
Target Species: Human, Non-Human Primates
Laser: Blue, Green, Yellow
Clonality: monoclonal
Field of Interest: Immunophenotyping
Source Species: Mouse
Regulatory Status: RUO
Clone: MEM-15
Order number: CA141309
HLDA Workshop HLDA III—WS Code M 252 HLDA IV—WS Code N 90 HLDA IV—WS Code T 53 HLDA V—WS Code... more
Product information "CyFlow™ CD14 PE-DyLight™ 594"
HLDA WorkshopHLDA III—WS Code M 252 HLDA IV—WS Code N 90 HLDA IV—WS Code T 53 HLDA V—WS Code M MA086 HLDA VI—WS Code M MA94
Quantity100 tests
Volume0.4 mL
ImmunogenA crude mixture of human urinary proteins precipitated by ammonium sulphate from the urine of a patient suffering from proteinuria
Background InformationCD14 (LPS-R) is a 55 kDa GPI-anchored glycoprotein, constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils, where serves as a multifunctional lipopolysaccharide receptor; it is also released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 is able to discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Safety Data Sheet

Technical Data Sheet

Instruction for Use

ECDoC

Certificate Of Analysis

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