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CyFlow™ CD20 PE-Cy7
| Application: | Flow cytometry |
| Excitation Maximum: | 496 nm, 565 nm |
| Clone: | 2H7 |
| Alternative Name: | B1, Bp35 |
| Antigen: | CD20 |
| Target Species: | Human, Non-Human Primates |
| Emission Maximum: | 785 nm |
| Format/Fluorochrome: | PE-Cy7 |
| Laser: | Blue, Green, Yellow |
| Clonality: | monoclonal |
| Field of Interest: | Immunophenotyping, Signaling |
| Source Species: | Mouse |
| Isotype: | IgG2b |
| Regulatory Status: | RUO |
| Order number: | BC091190 |
For Research Use Only
Not for use in diagnostic or therapeutic procedures.
HLDA Workshop HLDA IV—WS Code B201 Quantity 100 tests Volume 0.4 mL Immunogen... more
Product information "CyFlow™ CD20 PE-Cy7"
| HLDA Workshop | HLDA IV—WS Code B201 |
| Quantity | 100 tests |
| Volume | 0.4 mL |
| Immunogen | Human tonsillar B cells |
| Background Information | CD20 (B1, Bp35) is a cell surface 33-37 (depending on the degree of phosphorylation) kDa non-glycosylated surface phosphoprotein expressed on mature and most malignant B cells, but not stem cells or plasma cells (low number of the CD20 has been also detected on a subpopulation of T lymphocytes and it can be expressed on follicular dendritic cells). Its expression on B cells is synchronous with the expression of surface IgM. CD20 regulates transmembrane calcium conductance (probably functioning as a component of store-operated calcium channel), cell cycle progression and B-cell proliferation. It is associated with lipid rafts, but the intensity of this association depends on extracellular triggering, employing CD20 conformational change and/or BCR (B cell antigen receptor) aggregation. After the receptor ligation, BCR and CD20 colocalize and then rapidly dissociate before BCR endocytosis, whereas CD20 remains at the cell surface. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B cells, as it is expressed at high levels on most B-cell malignancies, but does not become internalized or shed from the plasma membrane following mAb treatment. |
| Usage | The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. |
| Storage Buffer | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
Safety Data Sheet
Technical Data Sheet
- CyFlow™ CD20 PE-Cy7 (2H7) AW819478 TDS US,CA EN.pdf
- CyFlow CD20 PE-Cy7 (2H7) BC091190 TDS RUO.pdf
- CyFlow CD20 PE-Cy7 (2H7) AW819478 TDS ASR EN.pdf
Instruction for Use
- CyFlow CD20 PE-Cy7 (2H7) BN996928 IFU CZ CZ.pdf
- CyFlow CD20 PE-Cy7 (2H7) CN828372 IFU CZ CZ.pdf
- CyFlow CD20 PE-Cy7 (2H7) CN828372 IFU DK DK.pdf
- CyFlow CD20 PE-Cy7 (2H7) CN828372 IFU FR FR.pdf
- CyFlow CD20 PE-Cy7 (2H7) CN828372 IFU GB EN.pdf
- CyFlow CD20 PE-Cy7 (2H7) CN828372 IFU GR EL.pdf
- CyFlow CD20 PE-Cy7 (2H7) CN828372 IFU IT IT.pdf
- CyFlow CD20 PE-Cy7 (2H7) CN828372 IFU NL NL.pdf
- CyFlow CD20 PE-Cy7 (2H7) CN828372 IFU NO NO.pdf
- CyFlow CD20 PE-Cy7 (2H7) CN828372 IFU PL PL.pdf
- CyFlow CD20 PE-Cy7 (2H7) CN828372 IFU RU RU.pdf
- CyFlow CD20 PE-Cy7 (2H7) CN828372 IFU SE SV.pdf
- CyFlow CD20 PE-Cy7 (2H7) CN828372 IFU SK SK.pdf
- CyFlow CD20 PE-Cy7 (2H7) CN828372 IFU TR TR.pdf
- CyFlow CD20 PE-Cy7 (2H7) CN828372 IFU DE DE.pdf
- CyFlow CD20 PE-Cy7 (2H7) BN996928 IFU DE DE.pdf
- CyFlow CD20 PE-Cy7 (2H7) BN996928 IFU DK DK.pdf
- CyFlow CD20 PE-Cy7 (2H7) BN996928 IFU ES ES.pdf
- CyFlow CD20 PE-Cy7 (2H7) BN996928 IFU FR FR.pdf
- CyFlow CD20 PE-Cy7 (2H7) BN996928 IFU GB EN.pdf
- CyFlow CD20 PE-Cy7 (2H7) BN996928 IFU GR EL.pdf
- CyFlow CD20 PE-Cy7 (2H7) BN996928 IFU IT IT.pdf
- CyFlow CD20 PE-Cy7 (2H7) BN996928 IFU NL NL.pdf
- CyFlow CD20 PE-Cy7 (2H7) BN996928 IFU NO NO.pdf
- CyFlow CD20 PE-Cy7 (2H7) BN996928 IFU PL PL.pdf
- CyFlow CD20 PE-Cy7 (2H7) BN996928 IFU PT PT.pdf
- CyFlow CD20 PE-Cy7 (2H7) BN996928 IFU RU RU.pdf
- CyFlow CD20 PE-Cy7 (2H7) BN996928 IFU SE SV.pdf
- CyFlow CD20 PE-Cy7 (2H7) BN996928 IFU SK SK.pdf
- CyFlow CD20 PE-Cy7 (2H7) BN996928 IFU TR TR.pdf
- CyFlow CD20 PE-Cy7 (2H7) CN828372 IFU ES ES.pdf
- CyFlow CD20 PE-Cy7 (2H7) CN828372 IFU PT PT.pdf
- CyFlow CD20 PE-Cy7 (2H7) BN996928 IFU BG BG.pdf
- CyFlow CD20 PE-Cy7 (2H7) BN996928 IFU HR HR.pdf
- CyFlow CD20 PE-Cy7 (2H7) BN996928 IFU RO RO.pdf
- CyFlow CD20 PE-Cy7 (2H7) BN996928 IFU SI SL.pdf
- CyFlow CD20 PE-Cy7 (2H7) BN996928 IFU RS SR.pdf
ECDoC
Certificate Of Analysis
- CyFow CD20 PE-Cy7_CN828372_COA_901609.pdf
- CyFlow CD20 PE-Cy7 AW819478 COA 902819.pdf
- CyFlow CD20 PE-Cy7 CN828372 COA 902819.pdf
- CyFlow CD20 PE-Cy7_AW819478_COA_901609.pdf
- CyFlow CD20 PE-Cy7 AW819478 COA 903736.pdf
- CyFlow CD20 PE-Cy7 BN996928 COA 905120.pdf
- CyFlow CD20 PE-Cy7 AW819478 COA 905120.pdf
- CyFlow CD20 PE-Cy7 AW819478 COA 906733.pdf
- CyFlow CD20 PE-Cy7 BN996928 COA 906733.pdf
- CyFlow CD20 PE-Cy7 AW819478 COA 909133.pdf
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