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CyFlow™ CD20 FITC
| Application: | Flow cytometry |
| Clonality: | monoclonal |
| Format/Fluorochrome: | FITC |
| Target Species: | Human |
| Regulatory Status: | RUO |
| Laser: | Blue |
| Excitation Maximum: | 490 to 495 nm |
| Emission Maximum: | 518 nm |
| Source Species: | Mouse |
| Field of Interest: | Immunophenotyping, Signaling |
| Isotype: | IgG2a |
| Antigen: | CD20 |
| Alternative Name: | B1, Bp35 |
| Clone: | LT20 |
| Order number: | CF552125 |
For Research Use Only
Not for use in diagnostic or therapeutic procedures.
Quantity 100 tests Volume 2.0 mL Immunogen Normal human lymphocytes from lymph node... more
Product information "CyFlow™ CD20 FITC"
| Quantity | 100 tests |
| Volume | 2.0 mL |
| Immunogen | Normal human lymphocytes from lymph node |
| Background Information | CD20 (B1, Bp35) is a cell surface 33-37 (depending on the degree of phosphorylation) kDa non-glycosylated surface phosphoprotein expressed on mature and most malignant B cells, but not stem cells or plasma cells (low number of the CD20 has been also detected on a subpopulation of T lymphocytes and it can be expressed on follicular dendritic cells). Its expression on B cells is synchronous with the expression of surface IgM. CD20 regulates transmembrane calcium conductance (probably functioning as a component of store-operated calcium channel), cell cycle progression and B-cell proliferation. It is associated with lipid rafts, but the intensity of this association depends on extracellular triggering, employing CD20 conformational change and/or BCR (B cell antigen receptor) aggregation. After the receptor ligation, BCR and CD20 colocalize and then rapidly dissociate before BCR endocytosis, whereas CD20 remains at the cell surface. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B cells, as it is expressed at high levels on most B-cell malignancies, but does not become internalized or shed from the plasma membrane following mAb treatment. |
| Usage | The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 20·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests. |
| Storage Buffer | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
Safety Data Sheet
Technical Data Sheet
- CyFlow™ CD20 FITC (2H7) CS695242 TDS US,CA EN.pdf
- CyFlow CD20 FITC (2H7) CR048468 TDS RUO.pdf
- CyFlow CD20 FITC (LT20) CF552125 TDS RUO.pdf
- CyFlow CD20 FITC (2H7) CS695242 TDS ASR EN.pdf
Instruction for Use
- CyFlow CD20 FITC (2H7) AU343178 IFU CZ CZ.pdf
- CyFlow CD20 FITC (2H7) AU343178 IFU DE DE.pdf
- CyFlow CD20 FITC (2H7) AU343178 IFU DK DK.pdf
- CyFlow CD20 FITC (2H7) AU343178 IFU ES ES.pdf
- CyFlow CD20 FITC (2H7) AU343178 IFU FR FR.pdf
- CyFlow CD20 FITC (2H7) AU343178 IFU GB EN.pdf
- CyFlow CD20 FITC (2H7) AU343178 IFU GR EL.pdf
- CyFlow CD20 FITC (2H7) AU343178 IFU IT IT.pdf
- CyFlow CD20 FITC (2H7) AU343178 IFU NL NL.pdf
- CyFlow CD20 FITC (2H7) AU343178 IFU NO NO.pdf
- CyFlow CD20 FITC (2H7) AU343178 IFU PT PT.pdf
- CyFlow CD20 FITC (2H7) AU343178 IFU RU RU.pdf
- CyFlow CD20 FITC (2H7) AU343178 IFU SE SV.pdf
- CyFlow CD20 FITC (2H7) AU343178 IFU SK SK.pdf
- CyFlow CD20 FITC (2H7) AU343178 IFU TR TR.pdf
- CyFlow CD20 FITC (2H7) AY003045 IFU CZ CZ.pdf
- CyFlow CD20 FITC (2H7) AU343178 IFU DE DE.pdf
- CyFlow CD20 FITC (2H7) AU343178 IFU PL PL.pdf
- CyFlow CD20 FITC (2H7) AY003045 IFU DE DE.pdf
- CyFlow CD20 FITC (2H7) AY003045 IFU DK DK.pdf
- CyFlow CD20 FITC (2H7) AY003045 IFU ES ES.pdf
- CyFlow CD20 FITC (2H7) AY003045 IFU FR FR.pdf
- CyFlow CD20 FITC (2H7) AY003045 IFU GB EN.pdf
- CyFlow CD20 FITC (2H7) AY003045 IFU GR EL.pdf
- CyFlow CD20 FITC (2H7) AY003045 IFU IT IT.pdf
- CyFlow CD20 FITC (2H7) AY003045 IFU NL NL.pdf
- CyFlow CD20 FITC (2H7) AY003045 IFU NO NO.pdf
- CyFlow CD20 FITC (2H7) AY003045 IFU PL PL.pdf
- CyFlow CD20 FITC (2H7) AY003045 IFU PT PT.pdf
- CyFlow CD20 FITC (2H7) AY003045 IFU RU RU.pdf
- CyFlow CD20 FITC (2H7) AY003045 IFU SE SV.pdf
- CyFlow CD20 FITC (2H7) AY003045 IFU SK SK.pdf
- CyFlow CD20 FITC (2H7) AY003045 IFU TR TR.pdf
- CyFlow CD20 FITC (2H7) AY003045 IFU BG BG.pdf
- CyFlow CD20 FITC (2H7) AY003045 IFU HR HR.pdf
- CyFlow CD20 FITC (2H7) AY003045 IFU RO RO.pdf
- CyFlow CD20 FITC (2H7) AY003045 IFU SI SL.pdf
- CyFlow CD20 FITC (2H7) AY003045 IFU RS SR.pdf
ECDoC
Certificate Of Analysis
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