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CyFlow™ CD25 PE

CyFlow™ CD25 PE
Application: Flow cytometry
Isotype: IgG1
Alternative Name: IL2Rα, p55, TAC
Antigen: CD25
Source Species: Rat
Field of Interest: Immunophenotyping, MHC
Target Species: Mouse
Emission Maximum: 576 nm
Clonality: monoclonal
Excitation Maximum: 496 nm, 565 nm
Laser: Blue, Green, Yellow
Format/Fluorochrome: PE
Regulatory Status: RUO
Clone: PC61.5
Order number: CX608435
Concentration Unit mg/mL Concentration 0,5 Quantity 0.1 mg Volume 0.2 mL... more
Product information "CyFlow™ CD25 PE"
Concentration Unitmg/mL
Concentration0,5
Quantity0.1 mg
Volume0.2 mL
ImmunogenB6.1 CTL cell line
Background InformationCD25 (IL2Rα, Tac) is a ligand-binding α subunit of interleukin 2 receptor (IL2R). Together with β and γ subunit CD25 constitues the high affinity IL2R, whereas CD25 alone serves as the low affinity IL2R. CD25 expression rapidly increases upon T cell activation. The 55 kDa CD25 molecule is enzymatically cleaved and shed from the cell surface as a soluble 45 kDa s-Tac, whose concentration in serum can be used as a marker of T cell activation. Expression of CD25 indicates the neoplastic phenotype of mast cells. CD25+ CD4+ FoxP3+ regulatory cells (Treg cells) play a crucial role in the control of organ-specific autoimmune diseases.
UsageThe reagent is designed for Flow Cytometry analysis. Suggested working usage is 2·µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Safety Data Sheet

Technical Data Sheet

Certificate Of Analysis

ECDoC

Instruction for Use

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