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CyFlow™ CD33 PerCP

CyFlow™ CD33 PerCP
Application: Flow cytometry
Target Species: Human, Non-Human Primates
Alternative Name: p67, SIGLECT-3
Antigen: CD33
Emission Maximum: 678 nm
Excitation Maximum: 482 to 490 nm
Format/Fluorochrome: PerCP
Isotype: IgG1
Clonality: monoclonal
Laser: Blue, Green
Field of Interest: Immunophenotyping
Source Species: Mouse
Regulatory Status: RUO
Clone: WM53
Order number: BE820817

For Research Use Only
Not for use in diagnostic or therapeutic procedures.

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Description
HLDA Workshop HLDA IV—WS Code M-505 Quantity 100 tests Volume 1.0 mL Immunogen... more
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Product information "CyFlow™ CD33 PerCP"
HLDA WorkshopHLDA IV—WS Code M-505
Quantity100 tests
Volume1.0 mL
ImmunogenHuman Acute Myelogenous Leukemia (AML) cells
Background InformationCD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies; these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
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Certificate Of Analysis

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