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CyFlow™ CD54 Alexa Fluor™ 700

CyFlow™ CD54 Alexa Fluor™ 700
Application: Flow cytometry
Clonality: monoclonal
Target Species: Human
Regulatory Status: RUO
Isotype: IgG2b
Source Species: Mouse
Field of Interest: Immunophenotyping
Laser: Red
Format/Fluorochrome: Alexa Fluor™ 700
Excitation Maximum: 702 nm
Emission Maximum: 723 nm
Antigen: CD54
Alternative Name: ICAM-1
Clone: 1H4
Order number: AE912016
Quantity 100 tests Volume 0.4 mL Immunogen Raji cells and spleen cells fused with NS1... more
Product information "CyFlow™ CD54 Alexa Fluor™ 700"
Application: Flow cytometry
Clonality: monoclonal
Target Species: Human
Regulatory Status: RUO
Isotype: IgG2b
Source Species: Mouse
Field of Interest: Immunophenotyping
Laser: Red
Format/Fluorochrome: Alexa Fluor™ 700
Excitation Maximum: 702 nm
Emission Maximum: 723 nm
Antigen: CD54
Alternative Name: ICAM-1
Clone: 1H4
Quantity100 tests
Volume0.4 mL
ImmunogenRaji cells and spleen cells fused with NS1 cells
Background InformationCD54 (ICAM-1) is a 90 kD member of the C2 subset of immunoglobulin superfamily. It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
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