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CyFlow™ CD58 FITC

CyFlow™ CD58 FITC
Antigen: CD58
Alternative Name: LFA-3
Clone: MEM-63
Application: Flow cytometry
Format/Fluorochrome: FITC
Laser: Blue
Target Species: Human, Pig
Field of Interest: Immunophenotyping
Species of Origin: Mouse
Regulatory Status: RUO
Clonality: monoclonal
Emission Maximum: 518 nm
Excitation Maximum: 490 to 495 nm
Isotype: IgG1
Order number: AU419943

For Research Use Only
Not for use in diagnostic or therapeutic procedures.

HLDA Workshop HLDA VI—WS Code AS A047 Quantity 100 tests Volume 2 Immunogen... more
CyFlow™ CD58 FITC
HLDA WorkshopHLDA VI—WS Code AS A047
Quantity100 tests
ImmunogenNALM-6 human pre-B cell line
Background InformationCD58 (LFA-3) is an immunoglobulin family adhession molecule expressed by both hematopoietic and non-hematopoietic cells (often on antigen presenting cells) and serving as ligand of CD2. This interaction is important for T cell-mediated immunity. CD58 is expressed in transmembrane form and in GPI-anchored form; the later is constitutively associated with protein kinases whereas the transmembrane form activates kinase activity upon triggering. CD58 is a powerful tool for detection of minimal residual disease in acute lymphocytic leukemia, and for evaluation of liver damage related with hepatitis B.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 20·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing Tris buffered saline (TBS) solution, pH ≈8.0, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Kishimoto T, Goyert S, Kikutani H, Mason D, Miyasaka M, Moretta L, Ohno T, Okumura K, Shaw S, Springer TA, Sugamura K, Sugawara H, von dem Borne AEGK, Zola H (Eds): Leucocyte Typing VI. Garland·Publishing·Inc,·New·York. 1997; 1‑1342. <·NLM·ID:·9712219·> | Bayas MV, Schulten K, Leckband D: Forced detachment of the CD2‑CD58 complex. Biophys·J. 2003·Apr; 84(4):2223‑33. <·PMID:·12668431·> | Veltroni M, De Zen L, Sanzari MC, Maglia O, Dworzak MN, Ratei R, Biondi A, Basso G, Gaipa G: I‑BFM‑ALL‑FCM‑MRD‑Study Group: Expression of CD58 in normal, regenerating and leukemic bone marrow B cells: implications for the detection of minimal residual disease in acute lymphocytic leukemia. Haematologica. 2003·Nov; 88(11):1245‑52. <·PMID:·14607753·> | Ariel O, Kukulansky T, Raz N, Hollander N: Distinct membrane localization and kinase association of the two isoforms of CD58. Cell·Signal. 2004·Jun; 16(6):667‑73. <·PMID:·15093607·> | Sheng L, Li J, Qi BT, Ji YQ, Meng ZJ, Xie M: Investigation on correlation between expression of CD58 molecule and severity of hepatitis B. World·J·Gastroenterol. 2006·Jul·14; 12(26):4237‑40. <·PMID:·16830383·>