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CyFlow™ CD62P APC

CyFlow™ CD62P APC
Application: Flow cytometry
Clonality: monoclonal
Target Species: Human, Non-Human Primates
Regulatory Status: RUO
Source Species: Mouse
Field of Interest: Immunophenotyping
Format/Fluorochrome: APC
Laser: Red
Excitation Maximum: 650 nm
Emission Maximum: 660 nm
Isotype: IgG1
Antigen: CD62P
Alternative Name: P-selectin
Clone: AK4
Order number: AV460497
HLDA Workshop HLDA VI—WS Code P-44 Quantity 100 tests Volume 1.0 mL Immunogen... more
Product information "CyFlow™ CD62P APC"
HLDA WorkshopHLDA VI—WS Code P-44
Quantity100 tests
Volume1.0 mL
ImmunogenHuman platelets
Background InformationCD62P (P-selectin) is an adhesion glycoprotein that is expressed on platelets and endothelial cells upon their activation. Interaction between CD62P and its mucin-like ligand PSGL-1 (P-selectin glycoprotein ligand-1) expressed on the microvilli of most leukocytes supports leukocyte rolling along postkapillary venules at the earliest time of inflammation. Both CD62P and PSGL-1 are extended glycoproteins that form homodimers. CD62P dimerization is probably mediated through interactions of the transmembrane domains and stabilizes leukocyte tethering and rolling, probably by increasing rebinding within a bond cluster.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
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