Welcome to
Sysmex Flow Cytometry Other European Country!

For ordering please make sure you are in your local shop.
If you are from Other European Country then please continue.
Otherwise please go to your country.

Go to my country Continue

Article successfully added.

CyFlow™ CD71 Biotin

CyFlow™ CD71 Biotin
Antigen: CD71
Alternative Name: T9, TFR, TRFR
Clone: MEM-75
Application: Flow cytometry
Format/Fluorochrome: Biotin
Target Species: Human
Field of Interest: Endocytosis, Immunophenotyping
Species of Origin: Mouse
Regulatory Status: RUO
Clonality: monoclonal
Isotype: IgG1
Order number: CF637400

For Research Use Only
Not for use in diagnostic or therapeutic procedures.

HLDA Workshop HLDA IV—WS Code A 45 HLDA V—WS Code T T-165 Concentration Unit mg/mL... more
CyFlow™ CD71 Biotin
HLDA WorkshopHLDA IV—WS Code A 45 HLDA V—WS Code T T-165
Concentration Unitmg/mL
Quantity0.1 mg
ImmunogenNALM-6 human pre-B cell line
Background InformationCD71 (transferrin receptor) is a type II transmembrane glycoprotein expressed as homodimer in erythroid blood cell line and in activated leukocytes. Upon binding of holotransferrin (complex of transferrin and iron ions), CD71 is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with CD71 and recycles to the cell surface, where it is released upon exposure to normal pH. CD71 is also involved in uptake of non-transferrin bound iron.
UsageThe reagent is designed for indirect immunofluorescence analysis by Flow Cytometry. Suggested working usage is 5·µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Knapp W, Dorken B, Gilks W, Rieber EP, Schmidt RE, Stein H, von dem Borne AEGK (Eds): Leucocyte Typing IV. Oxford·University·Press,·Oxford. 1989; 1‑1820. <·NLM·ID:·8914679·> | Takeichi M: Cadherin cell adhesion receptors as a morphogenetic regulator. Science. 1991·Mar·22; 251(5000):1451‑5. <·PMID:·2006419·> | Doussis IA, Gatter KC, Mason DY: CD68 reactivity of non‑macrophage derived tumours in cytological specimens. J·Clin·Pathol. 1993·Apr; 46(4):334‑6. <·PMID:·7684403·> | Grabbe S, Varga G, Beissert S, Steinert M, Pendl G, Seeliger S, Bloch W, Peters T, Schwarz T, Sunderkötter C, Scharffetter-Kochanek K: Beta2 integrins are required for skin homing of primed T cells but not for priming naive T cells. J·Clin·Invest. 2002·Jan; 109(2):183‑92. <·PMID:·11805130·> | Rouas-Freiss N, Moreau P, Ferrone S, Carosella ED: HLA‑G proteins in cancer: do they provide tumor cells with an escape mechanism?. Cancer·Res. 2005·Nov·15; 65(22):10139‑44. <·PMID:·16287995·> | Graham RM, Chua AC, Herbison CE, Olynyk JK, Trinder D: Liver iron transport. World·J·Gastroenterol. 2007·Sep·21; 13(35):4725‑36. <·PMID:·17729394·> | Beck Z, Balogh A, Kis A, Izsépi E, Cervenak L, László G, Bíró A, Liliom K, Mocsár G, Vámosi G, Füst G, Matko J: New cholesterol‑specific antibodies remodel HIV‑1 target cells' surface and inhibit their in vitro virus production. J·Lipid·Res. 2010·Feb; 51(2):286‑96. <·PMID:·19654424·>