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CyFlow™ CD71 Biotin

CyFlow™ CD71 Biotin
Application: Flow cytometry
Clonality: monoclonal
Target Species: Human
Regulatory Status: RUO
Source Species: Mouse
Field of Interest: Endocytosis, Immunophenotyping
Isotype: IgG1
Format/Fluorochrome: Biotin
Antigen: CD71
Alternative Name: T9, TFR, TRFR
Clone: MEM-75
Order number: CF637400
HLDA Workshop HLDA IV—WS Code A 045 HLDA V—WS Code T T-165 Concentration Unit mg/mL... more
Product information "CyFlow™ CD71 Biotin"
HLDA WorkshopHLDA IV—WS Code A 045 HLDA V—WS Code T T-165
Concentration Unitmg/mL
Concentration1
Quantity0.1 mg
Volume0.1 mL
ImmunogenNALM-6 human pre-B cell line
Background InformationCD71 (transferrin receptor) is a type II transmembrane glycoprotein expressed as homodimer in erythroid blood cell line and in activated leukocytes. Upon binding of holotransferrin (complex of transferrin and iron ions), CD71 is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with CD71 and recycles to the cell surface, where it is released upon exposure to normal pH. CD71 is also involved in uptake of non-transferrin bound iron.
UsageThe reagent is designed for indirect immunofluorescence analysis by Flow Cytometry. Suggested working usage is 5·µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
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