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CyFlow™ CD79a APC

CyFlow™ CD79a APC
Antigen: CD79a
Alternative Name: IgA, MB-1, MB1
Clone: HM47
Application: Flow cytometry
Format/Fluorochrome: APC
Laser: Red
Target Species: Chicken, Cow, Dog, Guinea pig, Horse, Human, Mouse, Non-Human Primates, Pig, Rabbit, Rat
Field of Interest: Immunophenotyping
Species of Origin: Mouse
Regulatory Status: RUO
Clonality: monoclonal
Emission Maximum: 660 nm
Excitation Maximum: 650 nm
Isotype: IgG1
Order number: AQ406223

For Research Use Only
Not for use in diagnostic or therapeutic procedures.

Quantity 100 tests Volume 1 Immunogen Synthetic peptide corresponding to C terminal... more
CyFlow™ CD79a APC
Quantity100 tests
ImmunogenSynthetic peptide corresponding to C terminal amino acids 208-222 of human CD79a
Background InformationCD79a (Ig α, MB1) forms disulfide-linked heterodimer with CD79b (Ig β, B29). They both are transmembrane proteins with extended cytoplasmic domains containing immunoreceptor tyrosine activation motives (ITAMs), and together with cell surface immunoglobulin they constitute B-cell antigen-specific receptor (BCR). CD79a and b are the first components of BCR that are expressed developmentally. They appear on pro-B cells in association with the endoplasmic reticulum chaperone calnexin. Subsequently, in pre-B cells, CD79 heterodimer is associated with λ5-VpreB surrogate immunoglobulin and later with antigen-specific surface immunoglobulins. At the plasma cell stage, CD79a is present as an intracellular component. CD79a/b complex interacts with Src-family tyrosine kinase Lyn, which phosphorylates its cytoplasmic ITAM motives to form docking sites for downstream signaling.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

| Mason DY, Cordell JL, Tse AG, van Dongen JJ, van Noesel CJ, Micklem K, Pulford KA, Valensi F, Comans-Bitter WM, Borst J, et al: The IgM‑associated protein mb‑1 as a marker of normal and neoplastic B cells. J·Immunol. 1991·Dec·1; 147(11):2474‑82. <·PMID:·1747162·> | Rassenti LZ, Kipps TJ: Expression of Ig‑beta (CD79b) by chronic lymphocytic leukemia B cells that lack immunoglobulin heavy‑chain allelic exclusion. Blood. 2000·Apr·15; 95(8):2725‑7. <·PMID:·10753858·> | Torlakovic E, Torlakovic G: B‑cell markers in lymphocyte predominance Hodgkin disease. Arch·Pathol·Lab·Med. 2002·Jul; 126(7):862‑3. <·PMID:·12123231·> | Islas-Ohlmayer M, Padgett-Thomas A, Domiati-Saad R, Melkus MW, Cravens PD, Martin Mdel P, Netto G, Garcia JV: Experimental infection of NOD/SCID mice reconstituted with human CD34+ cells with Epstein‑Barr virus. J·Virol. 2004·Dec; 78(24):13891‑900. <·PMID:·15564497·> | Fernandez NJ, West KH, Jackson ML, Kidney BA: Immunohistochemical and histochemical stains for differentiating canine cutaneous round cell tumors.. Vet·Pathol. 2005·Jul; 42(4):437‑45. <·PMID:·16006603·> | Bhargava P, Kallakury BV, Ross JS, Azumi N, Bagg A: CD79a is heterogeneously expressed in neoplastic and normal myeloid precursors and megakaryocytes in an antibody clone‑dependent manner. Am·J·Clin·Pathol. 2007·Aug; 128(2):306‑13. <·PMID:·17638667·> | Zhao XF, Hassan A, Perry A, Ning Y, Stass SA, Dehner LP: C‑MYC rearrangements are frequent in aggressive mature B‑Cell lymphoma with atypical morphology. Int·J·Clin·Exp·Pathol. 2008·Jan·1; 1(1):65‑74. <·PMID:·18784824·>