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CyFlow™ CD79a FITC

CyFlow™ CD79a FITC
HLDA Workshop HLDA V—WS Code BC cB018 HLDA VI—WS Code BP 193 HLDA VI—WS Code BP 89 HLDA VI—WS... more
CyFlow™ CD79a FITC
Antigen: CD79a
Alternative Name: IgA, MB-1, MB1
Clone: HM57
Application: Flow cytometry
Format/Fluorochrome: FITC
Laser: Blue
Target Species: Chicken, Cow, Guinea pig, Horse, Human, Mouse, Opossum, Pig, Rabbit, Rat
Field of Interest: Immunophenotyping
Species of Origin: Mouse
Regulatory Status: RUO
Clonality: monoclonal
Emission Maximum: 518 nm
Excitation Maximum: 490 to 495 nm
Isotype: IgG1
HLDA WorkshopHLDA V—WS Code BC cB018 HLDA VI—WS Code BP 193 HLDA VI—WS Code BP 89 HLDA VI—WS Code B B103 HLDA VI—WS Code B CD79.4
Quantity100 tests
ImmunogenSynthetic peptide corresponding to amino acids 202-216 of human CD79a
Background InformationCD79a (Ig α, MB1) forms disulfide-linked heterodimer with CD79b (Ig β, B29). They both are transmembrane proteins with extended cytoplasmic domains containing immunoreceptor tyrosine activation motives (ITAMs), and together with cell surface immunoglobulin they constitute B-cell antigen-specific receptor (BCR). CD79a and b are the first components of BCR that are expressed developmentally. They appear on pro-B cells in association with the endoplasmic reticulum chaperone calnexin. Subsequently, in pre-B cells, CD79 heterodimer is associated with λ5-VpreB surrogate immunoglobulin and later with antigen-specific surface immunoglobulins. At the plasma cell stage, CD79a is present as an intracellular component. CD79a/b complex interacts with Src-family tyrosine kinase Lyn, which phosphorylates its cytoplasmic ITAM motives to form docking sites for downstream signaling.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

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