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CyFlow™ CD80 PerCP

CyFlow™ CD80 PerCP
Application: Flow cytometry
Clonality: monoclonal
Target Species: Human
Regulatory Status: RUO
Laser: Blue, Green
Source Species: Mouse
Field of Interest: Immunophenotyping
Isotype: IgG1
Format/Fluorochrome: PerCP
Excitation Maximum: 482 to 490 nm
Emission Maximum: 678 nm
Antigen: CD80
Alternative Name: B7- B7-1, BB1
Clone: MEM-233
Order number: AP655226
Quantity 100 tests Volume 1.0 mL Immunogen Extracellular domain of human CD80 fused... more
Product information "CyFlow™ CD80 PerCP"
Quantity100 tests
Volume1.0 mL
ImmunogenExtracellular domain of human CD80 fused to human IgG1(Fc)
Background InformationCD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28 and of an inhibitory receptor CD152 (CTLA-4). Both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, the both molecules can also substitute for each other in this process. The question what are the differences in CD80 and CD86 competency has not been fully elucidated yet; there are still conflicts in results about their respective roles in initiation or sustaining of the T cell immune response.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
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