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CyFlow™ CD86 Low Endotoxin

CyFlow™ CD86 Low Endotoxin
Antigen: CD86
Alternative Name: B7-2, B70
Clone: BU63
Application: Flow cytometry, Functional assays, Immunohistochemistry (frozen sections), Immunoprecipitation, Western blot
Format/Fluorochrome: Low Endotoxin
Target Species: Human
Field of Interest: Immunophenotyping
Species of Origin: Mouse
Regulatory Status: RUO
Clonality: monoclonal
Isotype: IgG1
Order number: BL707204

For Research Use Only
Not for use in diagnostic or therapeutic procedures.

Concentration Unit mg/mL Concentration 1 Quantity 0.1 mg Volume 0.1 Immunogen... more
CyFlow™ CD86 Low Endotoxin
Concentration Unitmg/mL
Quantity0.1 mg
ImmunogenB-lymphoblastoid cell line ARH 77
Background InformationCD86 (B7-2) and CD80 (B7-1) are ligands of T cell critical costimulatory molecule CD28 and of an inhibitory receptor CD152 (CTLA-4). Both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, both molecules can also substitute for each other in this process. The question what are the differences in CD80 and CD86 competency has not been fully elucidated yet; there are still conflicts in results about their respective roles in initiation or sustaining of the T cell immune response.
Storage BufferThe reagent is provided in azide-free phosphate buffered saline (PBS) solution, pH ≈7.4; 0.2 µm filter sterilized. Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Doussis IA, Gatter KC, Mason DY: CD68 reactivity of non‑macrophage derived tumours in cytological specimens. J·Clin·Pathol. 1993·Apr; 46(4):334‑6. <·PMID:·7684403·> | Engel P, Gribben JG, Freeman GJ, Zhou LJ, Nozawa Y, Abe M, Nadler LM, Wakasa H, Tedder TF: The B7‑2 (B70) costimulatory molecule expressed by monocytes and activated B lymphocytes is the CD86 differentiation antigen. Blood. 1994·Sep·1; 84(5):1402‑7. <·PMID:·7520767·> | Caux C, Vanbervliet B, Massacrier C, Azuma M, Okumura K, Lanier LL, Banchereau J: B70/B7‑2 is identical to CD86 and is the major functional ligand for CD28 expressed on human dendritic cells. J·Exp·Med. 1994·Nov·1; 180(5):1841‑7. <·PMID:·7525840·> | Mauri D, Wyss-Coray T, Gallati H, Pichler WJ: Antigen‑presenting T cells induce the development of cytotoxic CD4+ T cells (I): Involvement of the CD80‑CD28 adhesion molecules. J·Immunol. 1995·Jul·1; 155(1):118‑27. <·PMID:·7541409·> | Kishimoto T, Goyert S, Kikutani H, Mason D, Miyasaka M, Moretta L, Ohno T, Okumura K, Shaw S, Springer TA, Sugamura K, Sugawara H, von dem Borne AEGK, Zola H (Eds): Leucocyte Typing VI. Garland·Publishing·Inc,·New·York. 1997; 1‑1342. <·NLM·ID:·9712219·> | Vasilevko V, Ghochikyan A, Holterman MJ, Agadjanyan MG: CD80 (B7‑1 and CD86 (B7‑2 are functionally equivalent in the initiation and maintenance of CD4+ T‑cell proliferation after activation with suboptimal doses of PHA. DNA·Cell·Biol. 2002·Mar; 21(3):137‑49. <·PMID:·12015893·> | Zhan H, Towler HM, Calder VL: The immunomodulatory role of human conjunctival epithelial cells. Invest·Ophthalmol·Vis·Sci. 2003·Sep; 44(9):3906‑10. <·PMID:·12939308·> | Giguere JF, Bounou S, Paquette JS, Madrenas J, Tremblay MJ: Insertion of host‑derived costimulatory molecules CD80 (B7.{01} and CD86 (B7.2) into human immunodeficiency virus type 1 affects the virus life cycle. J·Virol. 2004·Jun; 78(12):6222‑32. <·PMID:·15163715·> | Yadav D, Judkowski V, Flodstrom-Tullberg M, Sterling L, Redmond WL, Sherman L, Sarvetnick N: B7‑2 (CD86 controls the priming of autoreactive CD4 T cell response against pancreatic islets. J·Immunol. 2004·Sep·15; 173(6):3631‑9. <·PMID:·15356107·> | Chan A, Baird M, Mercer AA, Fleming SB: Maturation and function of human dendritic cells are inhibited by orf virus‑encoded interleukin‑10. J·Gen·Virol. 2006·Nov; 87(11):3177‑81. <·PMID:·17030850·> | Kolar GR, Mehta D, Pelayo R, Capra JD: A novel human B cell subpopulation representing the initial germinal center population to express AID. Blood. 2007·Mar·15; 109(6):2545‑52. <·PMID:·17132718·> | Thomas IJ, Petrich de Marquesini LG, Ravanan R, Smith RM, Guerder S, Flavell RA, Wraith DC, Wen L, Wong FS: CD86 has sustained costimulatory effects on CD8 T cells. J·Immunol. 2007·Nov·1; 179(9):5936‑46. <·PMID:·17947667·> | Eri R, Kodumudi KN, Summerlin DJ, Srinivasan M: Suppression of colon inflammation by CD80 blockade: evaluation of two murine models of inflammatory bowel disease. Inflamm·Bowel·Dis. 2008·Jan·9; <·PMID:·21061388·> | Hovden AO, Karlsen M, Jonsson R, Aarstad HJ, Appel S: Maturation of monocyte derived dendritic cells with OK432 boosts IL‑12p70 secretion and conveys strong T‑cell responses. BMC·Immunol. 2011·Jan·5; 12:2. <·PMID:·21208424·>