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CyFlow™ CD86 FITC

CyFlow™ CD86 FITC
Antigen: CD86
Alternative Name: B7-2, B70
Clone: GL-1
Application: Flow cytometry
Format/Fluorochrome: FITC
Laser: Blue
Target Species: Mouse
Field of Interest: Immunophenotyping, MHC
Species of Origin: Rat
Regulatory Status: RUO
Clonality: monoclonal
Emission Maximum: 518 nm
Excitation Maximum: 490 to 495 nm
Isotype: IgG2a
Order number: CQ603647

For Research Use Only
Not for use in diagnostic or therapeutic procedures.

Concentration Unit mg/mL Concentration 0.5 Quantity 0.1 mg Volume 0.2... more
CyFlow™ CD86 FITC
Concentration Unitmg/mL
Quantity0.1 mg
ImmunogenLPS-activated CBA/Cs mouse splenic B cells
Background InformationCD86 (B7-2) and CD80 (B7-1) are ligands of T cell critical costimulatory molecule CD28 and of an inhibitory receptor CD152 (CTLA-4). Both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, both molecules can also substitute for each other in this process. The question what are the differences in CD80 and CD86 competency has not been fully elucidated yet; there are still conflicts in results about their respective roles in initiation or sustaining of the T cell immune response.
UsageThe reagent is designed for Flow Cytometry analysis. Suggested working usage is 2·µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Hathcock KS, Laszlo G, Dickler HB, Bradshaw J, Linsley P, Hodes RJ: Identification of an alternative CTLA‑4 ligand costimulatory for T cell activation. Science. 1993·Nov·5; 262(5135):905‑7. <·PMID:·7694361·> | Benschop RJ, Melamed D, Nemazee D, Cambier JC: Distinct signal thresholds for the unique antigen receptor‑linked gene expression programs in mature and immature B cells. J·Exp·Med. 1999·Sep·20; 190(6):749‑56. <·PMID:·10499913·> | Brasel K, De Smedt T, Smith JL, Maliszewski CR: Generation of murine dendritic cells from flt3‑ligand‑supplemented bone marrow cultures. Blood. 2000·Nov·1; 96(9):3029‑39. <·PMID:·11049981·> | Chung JB, Wells AD, Adler S, Jacob A, Turka LA, Monroe JG: Incomplete activation of CD4 T cells by antigen‑presenting transitional immature B cells: implications for peripheral B and T cell responsiveness: implications for peripheral B and T cell responsiveness. J·Immunol. 2003·Aug·15; 171(4):1758‑67. <·PMID:·12902475·> | Steptoe RJ, Ritchie JM, Jones LK, Harrison LC: Autoimmune diabetes is suppressed by transfer of proinsulin‑encoding Gr‑1+ myeloid progenitor cells that differentiate in vivo into resting dendritic cells. Diabetes. 2005·Feb; 54(2):434‑42. <·PMID:·15677501·> | Nolan A, Weiden M, Kelly A, Hoshino Y, Hoshino S, Mehta N, Gold JA: CD40 and CD80/86 act synergistically to regulate inflammation and mortality in polymicrobial sepsis. Am·J·Respir·Crit·Care·Med. 2008·Feb·1; 177(3):301‑8. <·PMID:·17989345·> | Edgtton KL, Kausman JY, Li M, O'Sullivan K, Lo C, Hutchinson P, Yagita H, Holdsworth SR, Kitching AR: Intrarenal antigens activate CD4+ cells via co‑stimulatory signals from dendritic cells. J·Am·Soc·Nephrol. 2008·Mar; 19(3):515‑26. <·PMID:·18184859·> | Radhakrishnan S, Arneson LN, Upshaw JL, Howe CL, Felts SJ, Colonna M, Leibson PJ, Rodriguez M, Pease LR: TREM‑2 mediated signaling induces antigen uptake and retention in mature myeloid dendritic cells. J·Immunol. 2008·Dec·1; 181(11):7863‑72. <·PMID:·19017976·> | Nolan A, Kobayashi H, Naveed B, Kelly A, Hoshino Y, Hoshino S, Karulf MR, Rom WN, Weiden MD, Gold JA: Differential role for CD80 and CD86 in the regulation of the innate immune response in murine polymicrobial sepsis. PLoS·One. 2009·Aug·12; 4(8):e6600. <·PMID:·19672303·>