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CyFlow™ CD86 FITC

CyFlow™ CD86 FITC
Application: Flow cytometry
Clonality: monoclonal
Format/Fluorochrome: FITC
Regulatory Status: RUO
Laser: Blue
Excitation Maximum: 490 to 495 nm
Emission Maximum: 518 nm
Field of Interest: Immunophenotyping, MHC
Isotype: IgG2a
Target Species: Mouse
Source Species: Rat
Antigen: CD86
Alternative Name: B7-2, B70
Clone: GL-1
Order number: CQ603647
Concentration Unit mg/mL Concentration 0,5 Quantity 0.1 mg Volume 0.2 mL... more
Product information "CyFlow™ CD86 FITC"
Concentration Unitmg/mL
Concentration0,5
Quantity0.1 mg
Volume0.2 mL
ImmunogenLPS-activated CBA/Cs mouse splenic B cells
Background InformationCD86 (B7-2) and CD80 (B7-1) are ligands of T cell critical costimulatory molecule CD28 and of an inhibitory receptor CD152 (CTLA-4). Both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, both molecules can also substitute for each other in this process. The question what are the differences in CD80 and CD86 competency has not been fully elucidated yet; there are still conflicts in results about their respective roles in initiation or sustaining of the T cell immune response.
UsageThe reagent is designed for Flow Cytometry analysis. Suggested working usage is 2·µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
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