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CyFlow™ CD95 FITC

CyFlow™ CD95 FITC
Antigen: CD95
Alternative Name: APO-1, Fas, TNFRSF6
Clone: LT95
Application: Flow cytometry
Format/Fluorochrome: FITC
Laser: Blue
Target Species: Human
Field of Interest: Apoptosis, Immunophenotyping
Species of Origin: Mouse
Regulatory Status: RUO
Clonality: monoclonal
Emission Maximum: 518 nm
Excitation Maximum: 490 to 495 nm
Isotype: IgG1
Order number: BD855752

For Research Use Only
Not for use in diagnostic or therapeutic procedures.

Quantity 100 tests Volume 2 Immunogen HUT-78 human T cell lymphoma cell line... more
CyFlow™ CD95 FITC
Quantity100 tests
ImmunogenHUT-78 human T cell lymphoma cell line
Background InformationCD95 (Fas, APO-1), a 46 kDa transmembrane glycoprotein, is a cell death receptor of the TNFR superfamily. Stimulation of CD95 results in aggregation of its intracellular death domains, formation of the death-inducing signaling complex (DISC) and activation of caspases. In type I cells caspase 3 is activated by high amounts of caspase 8 generated at the DISC, in type II cells low concentration of caspase 8 activates pathway leading to the release of cytochrome c from mitochondria and activation of caspase 3 by cytochom c. Besides its roles in induction of apoptosis, Fas also triggers pro-inflammatory cytokine responses.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 20·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

| Scaffidi C, Fulda S, Srinivasan A, Friesen C, Li F, Tomaselli KJ, Debatin KM, Krammer PH, Peter ME: Two CD95 (APO‑1/Fas) signaling pathways. EMBO·J. 1998·Mar·16; 17(6):1675‑87. <·PMID:·9501089·> | Park DR, Thomsen AR, Frevert CW, Pham U, Skerrett SJ, Kiener PA, Liles WC: Fas (CD95 induces proinflammatory cytokine responses by human monocytes and monocyte‑derived macrophages. J·Immunol. 2003·Jun·15; 170(12):6209‑16. <·PMID:·12794152·> | Drosopoulos KG, Roberts ML, Cermak L, Sasazuki T, Shirasawa S, Andera L, Pintzas A: Transformation by oncogenic RAS sensitizes human colon cells to TRAIL‑induced apoptosis by up‑regulating death receptor 4 and death receptor 5 through a MEK‑dependent pathway. J·Biol·Chem. 2005·Jun·179 dec; 280(24):22856‑67. <·PMID:·15757891·> | Guo Z, Zhang M, Tang H, Cao X: Fas signal links innate and adaptive immunity by promoting dendritic‑cell secretion of CC and CXC chemokines. Blood. 2005·Sep·15; 106(6):2033‑41. <·PMID:·15941911·> | Brumatti G, Yon M, Castro FA, Bueno-da-Silva AE, Jacysyn JF, Brunner T, Amarante-Mendes GP: Conversion of CD95 (Fas) Type II into Type I signaling by sub‑lethal doses of cycloheximide. Exp·Cell·Res. 2008·Feb·1; 314(3):554‑63. <·PMID:·18078929·>