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CyFlow™ CD95 FITC
| Application: | Flow cytometry |
| Field of Interest: | Apoptosis, Immunophenotyping |
| Clone: | LT95 |
| Alternative Name: | APO-1, Fas, TNFRSF6 |
| Antigen: | CD95 |
| Isotype: | IgG1 |
| Source Species: | Mouse |
| Clonality: | monoclonal |
| Emission Maximum: | 518 nm |
| Excitation Maximum: | 490 to 495 nm |
| Laser: | Blue |
| Regulatory Status: | RUO |
| Target Species: | Human |
| Format/Fluorochrome: | FITC |
| Order number: | BD855752 |
For Research Use Only
Not for use in diagnostic or therapeutic procedures.
Quantity 100 tests Volume 2.0 mL Immunogen HUT-78 human T cell lymphoma cell line... more
Product information "CyFlow™ CD95 FITC"
| Quantity | 100 tests |
| Volume | 2.0 mL |
| Immunogen | HUT-78 human T cell lymphoma cell line |
| Background Information | CD95 (Fas, APO-1), a 46 kDa transmembrane glycoprotein, is a cell death receptor of the TNFR superfamily. Stimulation of CD95 results in aggregation of its intracellular death domains, formation of the death-inducing signaling complex (DISC) and activation of caspases. In type I cells caspase 3 is activated by high amounts of caspase 8 generated at the DISC, in type II cells low concentration of caspase 8 activates pathway leading to the release of cytochrome c from mitochondria and activation of caspase 3 by cytochom c. Besides its roles in induction of apoptosis, Fas also triggers pro-inflammatory cytokine responses. |
| Usage | The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 20·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests. |
| Storage Buffer | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
Safety Data Sheet
Technical Data Sheet
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