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CyFlow™ CD95 PE-Cy7

CyFlow™ CD95 PE-Cy7
Application: Flow cytometry
Isotype: IgG1
Clone: LT95
Alternative Name: APO-1, Fas, TNFRSF6
Antigen: CD95
Emission Maximum: 785 nm
Format/Fluorochrome: PE-Cy7
Excitation Maximum: 496 nm, 565 nm
Clonality: monoclonal
Laser: Blue, Green, Yellow
Field of Interest: Apoptosis, Immunophenotyping
Source Species: Mouse
Regulatory Status: RUO
Target Species: Human
Order number: CF494002
Quantity 100 tests Volume 0.4 mL Immunogen HUT-78 human T cell lymphoma cell line... more
Product information "CyFlow™ CD95 PE-Cy7"
Quantity100 tests
Volume0.4 mL
ImmunogenHUT-78 human T cell lymphoma cell line
Background InformationCD95 (Fas, APO-1), a 46 kDa transmembrane glycoprotein, is a cell death receptor of the TNFR superfamily. Stimulation of CD95 results in aggregation of its intracellular death domains, formation of the death-inducing signaling complex (DISC) and activation of caspases. In type I cells caspase 3 is activated by high amounts of caspase 8 generated at the DISC, in type II cells low concentration of caspase 8 activates pathway leading to the release of cytochrome c from mitochondria and activation of caspase 3 by cytochom c. Besides its roles in induction of apoptosis, Fas also triggers pro-inflammatory cytokine responses.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
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