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CyFlow™ CD117 PE-Cy5

CyFlow™ CD117 PE-Cy5
Application: Flow cytometry
Target Species: Cow, Human, Non-Human Primates
Alternative Name: c-kit, SCFR
Antigen: CD117
Field of Interest: Immunophenotyping, Phosphorylation
Emission Maximum: 670 nm
Format/Fluorochrome: PE-Cy5
Isotype: IgG1
Clonality: monoclonal
Excitation Maximum: 496 nm, 565 nm
Laser: Blue, Green, Yellow
Source Species: Mouse
Regulatory Status: RUO
Clone: 104D2
Order number: CH470016
HLDA Workshop HLDA VI—WS Code C-30 Quantity 100 tests Volume 0.4 mL Immunogen... more
Product information "CyFlow™ CD117 PE-Cy5"
HLDA WorkshopHLDA VI—WS Code C-30
Quantity100 tests
Volume0.4 mL
ImmunogenMOLM-1 megakaryocytic cells
Background InformationCD117 (c-Kit) is a 145 kDa receptor tyrosine kinase that regulates cell proliferation, adhesion, chemotaxis, apoptosis and other cell processes. Mutations of CD117 can lead to growth and progression of tumors. After binding of its ligand, SCF (stem cell factor), CD117 is autophosphorylated on its intracellular domains and activated. CD117 is expressed on pluripotent hematopoietic progenitor cells, mast cells and various cancer cells, e.g. acute myeloid leukemia cells.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
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