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CyFlow™ CD123 APC

CyFlow™ CD123 APC
Application: Flow cytometry
Clonality: monoclonal
Target Species: Human
Regulatory Status: RUO
Source Species: Mouse
Field of Interest: Immunophenotyping, Signaling
Format/Fluorochrome: APC
Laser: Red
Excitation Maximum: 650 nm
Emission Maximum: 660 nm
Isotype: IgG1
Antigen: CD123
Alternative Name: IL-3Ra, IL3R
Clone: 6H6
Order number: CF172111
Quantity 100 tests Volume 1.0 mL Immunogen IL3 receptor α chain expressed on the... more
Product information "CyFlow™ CD123 APC"
Quantity100 tests
Volume1.0 mL
ImmunogenIL3 receptor α chain expressed on the surface of transiently transfected COS cells
Background InformationCD123 is the α chain of interleukin 3 receptor (IL-3R α). This subunit heterodimerizes with the interleukin 3 receptor β chain (CD131), which is shared with other receptors. CD123 interacts with IL-3 specifically, but with low affinity, and association with the β subunit confers high affinity binding to the receptor heterodimer. Both chains are required for signaling, but receptor activation and signal transduction depend on IL-3 binding to CD123 as the initial step.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
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