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CyFlow™ CD135 FITC
| Application: | Flow cytometry |
| Field of Interest: | Immunophenotyping, Phosphorylation |
| Alternative Name: | Flk2, Flt3, STK1 |
| Antigen: | CD135 |
| Isotype: | IgG1 |
| Source Species: | Mouse |
| Clonality: | monoclonal |
| Emission Maximum: | 518 nm |
| Excitation Maximum: | 490 to 495 nm |
| Laser: | Blue |
| Regulatory Status: | RUO |
| Target Species: | Human |
| Format/Fluorochrome: | FITC |
| Clone: | BV10A4 |
| Order number: | AB955497 |
For Research Use Only
Not for use in diagnostic or therapeutic procedures.
Quantity 100 tests Volume 0.4 mL Immunogen BV-173 leukemic cell line Background... more
Product information "CyFlow™ CD135 FITC"
| Quantity | 100 tests |
| Volume | 0.4 mL |
| Immunogen | BV-173 leukemic cell line |
| Background Information | CD135 (FLT3, FLK2, STK-1) is a receptor tyrosine kinase that plays important roles in hematopoiesis. After binding of FLT3 ligand (FL), CD135 homodimerizes and stimulates proliferation, differentiation and protects the cell from apoptosis. The loss of CD90 and gain of CD135 expression marks the loss of self-renewal in hematopoietic stem cell population. Detectable CD135 expression appears first at low levels on the surface of primitive multilineage progenitor cells and disappears during defined stages of B-cell development but is upregulated and maintained during maturation of monocytes. CD135 is also expressed on thymocytes, dendritic cell progenitors and on mature dendritic cells, as well as on various malignant hematopoietic cells. |
| Usage | The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. |
| Storage Buffer | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
Safety Data Sheet
Technical Data Sheet
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