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CyFlow™ CD147 Alexa Fluor™ 488

CyFlow™ CD147 Alexa Fluor™ 488
Application: Flow cytometry
Emission Maximum: 519 nm
Alternative Name: 5F7, Basigin, BSG, EMMPRIN, M6, TCSF
Antigen: CD147
Excitation Maximum: 495 nm
Clonality: monoclonal
Format/Fluorochrome: Alexa Fluor™ 488
Isotype: IgG1
Field of Interest: Immunophenotyping
Source Species: Mouse
Laser: Blue
Regulatory Status: RUO
Target Species: Human
Clone: MEM-M6/1
Order number: AX229947
Quantity 100 tests Volume 0.4 mL Immunogen Protein A-CR purified soluble recombinant... more
Product information "CyFlow™ CD147 Alexa Fluor™ 488"
Quantity100 tests
Volume0.4 mL
ImmunogenProtein A-CR purified soluble recombinant form of CD147, CD147Rg, which consists of the cDNA coding for the hinge region, CH2-and CH3 domain of human IgG1 (CD147Rg is secreted by transfectants as a dimer)
Background InformationCD147 (basigin, neurothelin, OX-47, 5A11, CE9, M6) also known as EMMPRIN (extracellular matrix metalloproteinase inducer) or TCSF (tumour cell-derived collagenase-stimulatory factor) is an ubiquitously expressed cell surface protein with multiple glycosylated forms. The highest level of CD147 expression is on metabolically active cells, such as lymphoblasts, inflammatory cells, brown adipocytes and malignant tumor cells. CD147 has multiple functions, including facilitating of cell surface expression of monocarboxylate transporter proteins and extracellular matrix metalloproteinases, regulation of integrin functions, it plays roles in cell development and activation, fetal development or retinal function.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
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