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CyFlow™ CD150 FITC

CyFlow™ CD150 FITC
Application: Flow cytometry
Clonality: monoclonal
Format/Fluorochrome: FITC
Target Species: Human
Regulatory Status: RUO
Laser: Blue
Excitation Maximum: 490 to 495 nm
Emission Maximum: 518 nm
Source Species: Mouse
Field of Interest: Immunophenotyping
Isotype: IgG1
Antigen: CD150
Alternative Name: IPO-3, SLAMF1
Clone: SLAM.4
Order number: AW692255
Quantity 100 tests Volume 0.4 mL Immunogen Human CD150-transfected 300.19 cells... more
Product information "CyFlow™ CD150 FITC"
Quantity100 tests
Volume0.4 mL
ImmunogenHuman CD150-transfected 300.19 cells
Background InformationCD150 (SLAM; signaling lymphocyte activation molecule) is a 70-95 kDa single chain transmembrane phosphoglycoprotein of the CD2 family. Its extracellular part contains eight potential N-glycosylation sites, and the intracellular tail contains three unique tyrosine-based motifs. These binding sites can be recognized by SH2-binding phosphatases and the adaptor proteins, such as SAP/SH2D1A or EAT-2. The SLAM family receptors are involved in leukocytes activation and contribute to the effective germinal center formation, generation of high-affinity antibody-secreting plasma cells, and memory T and B cells, thereby facilitating long-term immune response. CD150 expression is upregulated after cell activation.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
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