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CyFlow™ CD154 FITC

CyFlow™ CD154 FITC
Application: Flow cytometry
Field of Interest: Immunophenotyping, MHC
Alternative Name: CD40L, gp39, IMD3, T-BAM, TNFSF5, TRAP-1
Antigen: CD154
Source Species: Hamster
Clonality: monoclonal
Target Species: Mouse
Isotype: IgG1
Emission Maximum: 518 nm
Excitation Maximum: 490 to 495 nm
Laser: Blue
Regulatory Status: RUO
Format/Fluorochrome: FITC
Clone: MR-1
Order number: CR720552
Concentration Unit mg/mL Concentration 0,5 Quantity 0.1 mg Volume 0.2 mL... more
Product information "CyFlow™ CD154 FITC"
Concentration Unitmg/mL
Concentration0,5
Quantity0.1 mg
Volume0.2 mL
ImmunogenActivated mouse Th1 clone D1.6
Background InformationCD154 / CD40L (CD40 ligand) is a member of the tumor necrosis factor family, and is expressed primarily on activated CD4+ lymphocytes, but also on mast cells, basophils, eosinophils and human dendritic cells. Its counter-receptor CD40 is expressed on antigen presenting cells, including dendritic cells, macrophages, and B cells, and also on fibroblasts. Triggering of CD40 by CD40L causes maturation of dendritic cells and upregulation of antigen presentation in functions of the MHC and costimulatory molecules. CD40L also functions as a direct stimulating factor for T cells. CD40L plays also roles e.g. in antibody class switching and modulation of apoptosis in the germinal center.
UsageThe reagent is designed for Flow Cytometry analysis. Working concentrations should be determined by the investigator.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
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