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CyFlow™ CD203c FITC

CyFlow™ CD203c FITC
Application: Flow cytometry
Isotype: IgG1
Alternative Name: B10, E-NPP3, NPP3, PD-1b, PDNP3
Antigen: CD203c
Field of Interest: Immunophenotyping
Clonality: monoclonal
Source Species: Mouse
Emission Maximum: 518 nm
Excitation Maximum: 490 to 495 nm
Laser: Blue
Regulatory Status: RUO
Target Species: Human
Format/Fluorochrome: FITC
Clone: NP4D6
Order number: CT441730
HLDA Workshop HLDA VIII Quantity 100 tests Volume 0.4 mL Immunogen HEK-293 cells... more
Product information "CyFlow™ CD203c FITC"
HLDA WorkshopHLDA VIII
Quantity100 tests
Volume0.4 mL
ImmunogenHEK-293 cells transfected with human CD203c
Background InformationCD203c (ENPP-3) is integral membrane ectoenzyme (ectonucleotide pyrophosphatase/phosphodiesterase 3), that hydrolyses nucleotide triphosphates and thus modulates purinergic signaling. CD203c is expressed mainly on activated basophils and mast cells. CD203c is upregulated in response to IgE-receptor cross-linking and is overexpressed on neoplastic mast cells in patients with systemic mastocytosis. Measurement of its induced enhancement on the plasma membrane is useful for diagnostics of allergies.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
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