CyFlow™ ABRA1 Purified
| Application: | Flow cytometry, Immunoprecipitation, Western blot |
| Clonality: | monoclonal |
| Target Species: | Human |
| Regulatory Status: | RUO |
| Source Species: | Mouse |
| Format/Fluorochrome: | Purified |
| Isotype: | IgG |
| Antigen: | ABRA1 |
| Clone: | ABRA1-01 |
| Field of Interest: | Adaptor proteins, Transcription |
| Order number: | AP667270 |
For Research Use Only
Not for use in diagnostic or therapeutic procedures.
| Concentration Unit | mg/mL |
| Concentration | 1 |
| Quantity | 0.1 mg |
| Volume | 0.1 mL |
| Immunogen | Recombinant protein corresponding to amino acids 1-313 of ABRA1 with N-terminal His6 tag |
| Background Information | ABRA1 (Abraxas, CCDC98) is an adaptor protein that is essential for formation and function of BRCA1 A tumor suppressor complex. This complex plays critical roles in DNA repair, cell cycle checkpoint control, and maintenance of genomic stability. ABRA1 mediates interaction of ubiquitin-interacting motif-containing protein RAP80 and deubiquitination enzyme BRCC36 with BRCA1/BARD1. ABRA1 controls both DNA-damage-induced formation of BRCA1 foci and BRCA1-dependent G2/M checkpoint activation. |
| Storage Buffer | The reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
| Wang B, Matsuoka S, Ballif BA, Zhang D, Smogorzewska A, Gygi SP, Elledge SJ: Abraxas and RAP80 form a BRCA1 protein complex required for the DNA damage response. Science. 2007·May·25; 316(5828):1194‑8. <·PMID:·17525340·> | Liu Z, Wu J, Yu X: CCDC98 targets BRCA1 to DNA damage sites. Nat·Struct·Mol·Biol. 2007·Aug; 14(8):716‑20. <·PMID:·17643121·> | Wang B, Elledge SJ: Ubc13/Rnf8 ubiquitin ligases control foci formation of the Rap80/Abraxas/Brca1/Brcc36 complex in response to DNA damage. Proc·Natl·Acad·Sci·USA. 2007·Dec·26; 104(52):20759‑63. <·PMID:·18077395·> | Nikkilä J, Coleman KA, Morrissey D, Pylkäs K, Erkko H, Messick TE, Karppinen SM, Amelina A, Winqvist R, Greenberg RA: Familial breast cancer screening reveals an alteration in the RAP80 UIM domain that impairs DNA damage response function. Oncogene. 2009·Apr·23; 28(16):1843‑52. <·PMID:·19305427·>