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CyFlow™ Helios PE
Application: | Flow cytometry |
Emission Maximum: | 576 nm |
Alternative Name: | IKZF2 |
Antigen: | Helios |
Field of Interest: | Transcription |
Source Species: | Hamster |
Isotype: | IgG |
Target Species: | Human, Mouse |
Excitation Maximum: | 496 nm, 565 nm |
Clonality: | monoclonal |
Laser: | Blue, Green, Yellow |
Format/Fluorochrome: | PE |
Regulatory Status: | RUO |
Clone: | 22F6 |
Order number: | CV254145 |
For Research Use Only
Not for use in diagnostic or therapeutic procedures.
Quantity 100 tests Volume 1.0 mL Immunogen Peptide coresponding to the amino acids... more
Product information "CyFlow™ Helios PE"
Quantity | 100 tests |
Volume | 1.0 mL |
Immunogen | Peptide coresponding to the amino acids 51-107 of Helios |
Background Information | Helios (IKZF2; Ikaros family zinc finger protein 2) is a hematopoietic-specific transcription factor involved in the regulation of lymphocyte development, together with other members of this family, such as Aiolos and Ikaros. Helios forms homo- and heterodimers with these proteins and is thought to function predominantly in early hematopoietic development. Expression of Helios, Aiolos and Ikaros is restricted to cells of the hematopoietic system, whereas other family members, Eos and Pegassus, are more widely expressed. Helios is expressed at early stages of thymocyte development. In mature T cells, Helios has been strongly associated with Treg cells. |
Usage | The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. |
Storage Buffer | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA. |
Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
Stability | Do not use after expiration date stamped on vial label. |
Safety Data Sheet
Technical Data Sheet
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