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CyFlow™ IgE FITC

CyFlow™ IgE FITC
Application: Flow cytometry
Clonality: monoclonal
Format/Fluorochrome: FITC
Target Species: Human
Regulatory Status: RUO
Laser: Blue
Excitation Maximum: 490 to 495 nm
Emission Maximum: 518 nm
Source Species: Mouse
Isotype: IgG1
Field of Interest: Immunoglobulins
Antigen: IgE
Clone: BE5
Order number: CM987907
Concentration Unit mg/mL Concentration 0,1 Quantity 0.1 mg Volume 1.0 mL... more
Product information "CyFlow™ IgE FITC"
Concentration Unitmg/mL
Concentration0,1
Quantity0.1 mg
Volume1.0 mL
ImmunogenPurified human IgE
Background InformationImmunoglobulin E (IgE) is a 180 kDa soluble protein serving as an antigen-specific unit of mast cell effector mechanisms. IgE has the lowest serum concentration of all immunoglobulins (approximately 0.5 mg/l) in healthy individuals, but upon allergen challenge its concentration in blood increases dramatically. Although biological survival of free IgE is very short (T1/2 = 2 days), it is stabilized after binding to its high affinity receptor. Unlike IgM- IgG- and IgA-committed B cells, IgE-switched B cells do not undergo clonal expansion.
UsageThe reagent is designed for Flow Cytometry analysis. Suggested working usage is 5·µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
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