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CyFlow™ MHCII FITC

CyFlow™ MHCII FITC
Application: Flow cytometry
Clonality: monoclonal
Format/Fluorochrome: FITC
Regulatory Status: RUO
Isotype: IgG2b
Laser: Blue
Excitation Maximum: 490 to 495 nm
Emission Maximum: 518 nm
Field of Interest: Immunophenotyping, MHC
Target Species: Mouse
Source Species: Rat
Antigen: MHC Class II
Clone: M5/114
Order number: BJ649436
Concentration Unit mg/mL Concentration 0,5 Quantity 0.1 mg Volume 0.2 mL... more
Product information "CyFlow™ MHCII FITC"
Concentration Unitmg/mL
Concentration0,5
Quantity0.1 mg
Volume0.2 mL
ImmunogenActivated C57BL/6 mouse spleen cells
Background InformationMHC (major histocompatibility complex) class II (MHCII) molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells. Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells. The antigen-binding groove between MHC class II α and β chain is open at both ends and is 15-24 amino acid residues long.
UsageThe reagent is designed for Flow Cytometry analysis. Suggested working usage is 4·µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
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