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CyFlow™ Beta2-MG Alexa Fluor™ 647

Antigen:	β2-microglobulin
Clone:	B2M-01
Application:	Flow cytometry
Format/Fluorochrome:	Alexa Fluor™ 647
Laser:	Red
Target Species:	Human
Field of Interest:	Blood group antigens, MHC
Species of Origin:	Mouse
Regulatory Status:	RUO
Clonality:	monoclonal
Emission Maximum:	665 nm
Excitation Maximum:	650 nm
Isotype:	IgG2a

Concentration Unit	mg/mL
Concentration	1
Quantity	0.1 mg
Volume	0.1
Immunogen	Purified human β2-microglobulin
Background Information	β2-microglobulin (β2M) non-covalently associates with the 44 kDa α chain to forms the HLA Class I antigen complex. Human β2-microglobulin associated with HLA Class I antigens is expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.
Usage	The reagent is designed for Flow Cytometry analysis. Suggested working usage is 2·µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.
Storage Buffer	The reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
Storage	Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
Stability	Do not use after expiration date stamped on vial label.

Specific References

| Hilgert I, Horejsi V, Kristofova H: The use of murine monoclonal antibody B2M‑01 for detection and purification of human beta 2‑microglobulin. Folia·Biol·(Praha). 1984; 30(6):369‑76. <·PMID:·6394369·> | Lafon M, Prehaud C, Megret F, Lafage M, Mouillot G, Roa M, Moreau P, Rouas-Freiss N, Carosella ED: Modulation of HLA‑G Expression in Human Neural Cells after Neurotropic Viral Infections. J·Virol. 2005·Dec; 79(24):15226‑15237. <·PMID:·16306594·> | Khurana M, Traum AZ, Aivado M, Wells MP, Guerrero M, Grall F, Libermann TA, Schachter AD: Urine proteomic profiling of pediatric nephrotic syndrome. Pediatr·Nephrol. 2006·Sep; 21(9):1257‑65. <·PMID:·16810512·> | Poláková K, Železníková T, Russ G: HLA‑G5 in the blood of leukemia patients and healthy individuals. Leuk·Res. 2013·Feb; 37(2):139‑45. <·PMID:·23146557·>